EIDC
Leitão, A.B.; Ramesh, A.; Day, J.P.; Hanna, N.; Devi, A.; Hayes, M.P.; Jiggins, F.M.

Changes in gene expression and encapsulation in Drosophila melanogaster caused by exposure to parasitoid wasps

https://doi.org/10.5285/2998b066-6a35-4e4f-ae39-16838781856b Cite this dataset
Data consists of gene expression estimates and encapsulation rates in wild caught Drosophila melanogaster larvae following exposure to different treatments. Treatments include injection with wasp homogenate, injection with oil and no injection. Also provided are functional enrichment categories for differentially expressed genes and library generation and read mapping metrics.

The data were produced under the grant: NE/P00184X/1
Publication date: 2021-10-01

Formats

Comma-separated values (CSV)
txt

Temporal information

Temporal extent
2017-10-01    to    2017-10-31

Provenance & quality

Drosophila melanogaster larvae obtained from a wild caught population were exposed injected with wasp homogenate, oil or no manipulation was made. Encapsulation rates were measured in each case in the fly larvae. For RNA-sequencing, hemocyte and fat body tissues were isolated from third instar larvae 24 hours post experimental manipulation. RNA was isolated for sequencing. Sequenced reads were trimmed, mapped to the dm6 genome, number of reads mapping to each gene was counted, differential expression tests were applied and functional enrichment was done.

Correspondence/contact details

Ramesh, A.
University of Cambridge
 ar885@cam.ac.uk

Authors

Leitão, A.B.
University of Cambridge
ORCID iD icon https://orcid.org/0000-0001-9004-1831
Ramesh, A.
University of Cambridge
ORCID iD icon https://orcid.org/0000-0003-0050-3516
Day, J.P.
University of Cambridge
Hanna, N.
University of Cambridge
Devi, A.
University of Cambridge
Hayes, M.P.
University of Cambridge
Jiggins, F.M.
University of Cambridge
ORCID iD icon https://orcid.org/0000-0001-7470-8157

Other contacts

Rights holder
University of Cambridge
Custodian
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk

Additional metadata

Topic categories
biota
Keywords
differential gene expression , Drosophila melanogaster , encapsulation , encapsulation , Evolutionary genetics , fat body , genetics , haemocyte , insect , insect genomics , Leptopilina boulardi , parasite , parasitoid wasp , RNA-sequencing
Funding
Natural Environment Research Council Award: NE/P00184X/1
Last updated
08 February 2024 17:34

Get the data

THIS DATASET HAS BEEN SUPERSEDED. The latest version is Changes in gene expression and encapsulation in Drosophila melanogaster caused by exposure to parasitoid wasps v2

We relaxed the threshold for filtering out lowly expressed genes. Here we include genes that had counts per million > 2 in at least 4 out of 12 libraries, performed separately for fat body and hemocytes. This replaces the more stringent filter of counts per million > 10 in at least 4 libraries that was applied previously.

If you need access to this archived version, please contact the EIDC

CITE AS: Leitão, A.B.; Ramesh, A.; Day, J.P.; Hanna, N.; Devi, A.; Hayes, M.P.; Jiggins, F.M. (2021). Changes in gene expression and encapsulation in Drosophila melanogaster caused by exposure to parasitoid wasps. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/2998b066-6a35-4e4f-ae39-16838781856b